PřF:C5850 Intro to Biophysical Chemistry - Course Information

C5850 Introduction to Biophysical Chemistry

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching

Teacher(s)

doc. RNDr. Mgr. Jozef Hritz, Ph.D. (lecturer)
RNDr. Mgr. Iveta Třísková, Ph.D. (lecturer)

Guaranteed by

doc. RNDr. Mgr. Jozef Hritz, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science

Prerequisites

Absolutorim from physical chemistry and biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Biophysical Chemistry (programme PřF, B-CH)
• Physical Chemistry (programme PřF, N-CH)

Course objectives

1. Strategy and tactics of biophysical chemistry (basic questions and strategic approaches of biophysical chemistry, structural features of biopolymers, main role of CO2 and water in biological systems)

2. Some problems concerning space structure of biopolymers (tertiary and quaternary structure of biopolymers, stability of helical structures, hydrogen and disulphidic bonds, hydrophobic interactions)

3. Main determinates of biomolecular shape (Ramachandran plot, trans-cis preference of aminoacids, specific role of proline, hydrophobic interactions, distribution of aminoacids over protein structure)

4. Size and shape of macromolecules in solution (statistical interpretation of polymer solutions, dialysis, osmotic pressure, hydrodynamic methods, diffusion)

5. Canonical structural ensemble (Boltzmann probability, Gibbs and Helmholtz free energy, enthalpy, statistical interpretation of entropy)

6. Thermodynamics of biological systems (1) (stability of protein elements, folding and denaturation of biopolymers, first and second order phase transitions)

7. Thermodynamics of biological systems (2) (thermodynamics of biological processes, Molten-Globule state of proteins, formalism of non-equilibrium thermodynamics)

8. Polyelectrolytes (PEL) (theory of PEL at limiting concentration, distribution of counterions, expansion of polyions, thermodynamics, transport and electrical behavior of PEL, models of PEL)

9. Kinetics of biological systems (kinetic processes with several substrates and intermediates, catalysis, enzymatic reactions and regulation of them, biological clocks)

10. Polarography and voltammetry of biological molecules (use of polarographic and voltammetric techniques in research of electrochemical behaviour of proteins, nucleic acids and its components; analysis of pharmaceutical important molecules)

11. Electron and ions transfer processes in biological systems

Learning outcomes

Deeper application potential of physical chemistry to biological systems.

Syllabus

• Strategy and Tactics of Biophysical Chemistry.
  Some problems concerning space structure of biopolymers.
  Interaction of Biological Molecules with Electromagnetic Radiation.
  Size and Shape of Macromolecules.
  Thermodynamics of Biological Systems.
  Statistical Interpretation of Polymer Solution.
  Behaviour of Macromolecules in Solution.
  Polyelectrolytes.
  Kinetics of Biological Systems.
  Polarography and Voltammetry of Biological Molecules.
  Electron and Ion Transfer Processes in Biological System.

Literature

required literature
• KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. 2., přeprac. a rozš. vyd. Praha: Academia, 2000, 337 s. ISBN 8020007911. info
• CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part II, Techniques for the study of biological structure and function. 12th print. New York: W.H. Freeman and Company, 2001, xxix, s. 3. ISBN 0-7167-1189-3. info
• CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part I, The conformation of biological macromolecules. 11th print. New York: W.H. Freeman and Company, 1999, xxii, 341. ISBN 0-7167-1042-0. info
• CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part III, The behavior of biological macromolecules. New York: W.H. Freeman and Company, 1980, xxix, s. 8. ISBN 0-7167-1191-5. info

recommended literature
• KALOUS, Vítěz and Zdeněk PAVLÍČEK. Biofyzikální chemie. 1. vyd. Praha: Nakladatelství technické literatury, 1980, 349 s. info
• Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
• H. Eisenberg: Biological Macromolecules and Polyelectrolytes in Solutions, Clarendon Press, Oxford 1976
• MORAWETZ, Herbert. Chování makromolekul v roztoku. Vyd. 1. Praha: Academia, nakladatelství Československé akademie věd, 1971, 512 s. info

not specified
• FINKEL‘ŠTEJN, Aleksej Vital‘jevič and Oleg Borisovič PTICYN. Protein physics : a course of lectures. Amsterdam: Academic Press, 2002, xix, 354. ISBN 0122567811. info

Teaching methods

lectures, discussion, theoretical training, ppt presentation, solution of typical examples

Assessment methods

two written tests and oral examination

Language of instruction

Czech

Follow-Up Courses

• C5855 Methods in Biophysical Chemistry

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

• Enrolment Statistics (recent)
  
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