C6230 Clinical Biochemistry - laboratory course

Faculty of Science
Autumn 2025
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: RNDr. Jitka Kašparovská, Ph.D.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
C6221 Clinical Biochemistry ||NOW( C6221 Clinical Biochemistry )
Předpokladem zápisu je současné absolvování předmětu klinická biochemie (C6220). K zápisu je potřeba požádat o výjimku-souhlas učitele. Souhlas bude udělován v pořadí: Aplikovaná biochemie (předmět je povinný), Biochemie (předmět je povinně volitelný), ostatní.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 48 student(s).
Current registration and enrolment status: enrolled: 0/48, only registered: 0/48, only registered with preference (fields directly associated with the programme): 0/48
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the tests used in the examination of basic analytes in blood plasma/serum or urine, their principles and basic methods of interpretation of the obtained results.
Learning outcomes
After completing the course, the student will:
  • be familiar with the basic principles of working with potentially infectious biological material;
  • be able to explain the meaning and principles of examination of basic parameters in plasma/serum and urine;
  • know the physiological values ​​of the common biochemical parameters and able be to explain the basic causes leading to their change;
  • be able to interpret the results in the health/disease relationship.
    • Syllabus
    • Principles of laboratory biosafety and biosecurity.
    • Blood/urine collection and processing. Interpretation of results. Biochemical analyzers.
    • Examination of lipid and cholesterol metabolism: S/B-cholesterol, S-TG, S-LDL/HDL/non-HDL-cholesterol.
    • Examination of glucose metabolism: P/B/U-glucose, OGTT, B-HbA1.
    • Determination of proteins: S-total protein, S-albumin, electrophoresis, S/B-CRP, ELISA.
    • Determination of enzyme activity: S-ALT, S-AST, S-ALP isoenzymes, S-CK-MBmass.
    • Examination of nitrogen metabolites: S-bilirubin, S/U-urea, nitrogen balance.
    • Renal examination I: U-proteins and proteinurias, U-albumin. Qualitative urine analysis with diagnostic strips.
    • Renal examination II: U-osmolality, S/U-creatinine, creatinine clearance, eGFR, FR and FE of water.
    • Urolithiasis: chemical analysis of urinary calculi, S/U-uric acid, S/U-calcium.
    • GIT examination: Sal-lactate, HCl excretion, detection of Helicobacter pylori, S-amylase, secretin-CCK test, NBT-PABA test, MTG breath test, FOBT.
    Literature
      required literature
    • Interaktivní osnova v IS MU.
      recommended literature
    • RACEK, Jaroslav and Daniel RAJDL. Klinická biochemie. Třetí, přepracované a ro. Praha: Galén, 2021, 454 stran. ISBN 9788074925450. info
    Teaching methods
    Lab courses, discussion.
    Assessment methods
    Credit. Conditions for granting credit:
    1) completion of all exercises, 2) submission of lab reports, 3) completion of progress tests.
    Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    The course is taught every week.
    Teacher's information
    White coat, slippers or sturdy shoes with covers, gloves, and a calculator are required for the exercise.

    Detailed information about the course and course completion is given in the Study Materials in IS MU.

    The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
    • Enrolment Statistics (Autumn 2025, recent)
    • Permalink: https://is.muni.cz/course/sci/autumn2025/C6230