PřF:C5910 Chromatographic Methods I. - Course Information
C5910 Chromatographic Methods I.
Faculty of ScienceAutumn 1999
- Extent and Intensity
- 2/0/0. 4 credit(s). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Aleš Hrdlička, CSc. - Prerequisites
- HPLC/MS is discussed in C5900 course, Mass Spectrometry.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Ecotoxicology (programme PřF, M-BI)
- Ecotoxicology (programme PřF, N-BI)
- Environmental Chemistry (programme PřF, M-CH)
- Environmental Chemistry (programme PřF, N-CH)
- Syllabus
- I. Chromatographic separation, chromatogram, separation efficiency, performance. Broadening. II. Liquid chromatography II.1 HPLC, Stationary and mobile phases. II.2 Column in HPLC II.3 Mobile phase - Solvent classification, mixtures, Optimization, elution. II.4 HPLC techniques - Principles, retention models, separation strategy, applications. II.5 Instrumentation II.6 Methods of quantitative analysis II.7 Sample preparation, Multidimensional chromatography, Microcolumns, High speed HPLC. III. Thin Layer Chromatography: HPTLC, Technique, Quantitation, Instrumentation, Applications.
- Assessment methods (in Czech)
- přednášky, ústní zkouška
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (Autumn 1999, recent)
- Permalink: https://is.muni.cz/course/sci/autumn1999/C5910