B9080 Technical processes in biotechnology

Faculty of Science
Autumn 2001
Extent and Intensity
2/1/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Prerequisites (in Czech)
Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Similarity of systems and processes. Diffusion. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
Literature
  • 1. Ledersen B.K., D´Elia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
Assessment methods (in Czech)
Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 1999, Autumn 2000.
  • Enrolment Statistics (recent)
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