Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Mon 11:00–12:50 Bpt,01013
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2006, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2006/Bi7722