C7940 Bioanalytics I - Biomacromolecules

Faculty of Science
Autumn 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Tue 12:00–13:50 Kontaktujte učitele
Prerequisites
Basic knowledge of biochemistry is prerequisited (C3181 Biochemistry I, C4182 Biochemistry II). C7895 Mass Spectrometry of biomolecules is a recommended lecture
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Subject summarises the basic knowledge of the analytical approaches in analysis of biomacromolecules snd suprabiomolecular structures and also important theoretical background for their understanding.
Syllabus
  • 1) analytical approaches in v bioanalytics – demands; high throuput, shotgun analysis, standardization
  • afinity methods – principles, carry-out and use (separation, interaction studies), IMAC, APAGE
  • 2) immunoanalytic methods – principles; immunoanalysis – precipitation, agglutination, immunoessay, methods: FIA, LIA, RIA, EIA, ELISA, IAC, IACE, SELDI…
  • 3) gel electrophoresis – principles, carry-out (1D, 2D); blotting
  • 4) genomics (analysis of DNA) – fundaments of genetics (structure, function of DNA), analytical approaches: PCR, restriction enzymes; direct and indirect sequencing; DNA methylation and its analysis
  • 5) basics of mass spectrometry – principles; ionisation, mass analysis (tandem MS, vacuum technique), detection; mass spectrum
  • proteomics (analysis of proteins and their complexes) – fundamentals (structure of proteins and protein complexes)
  • expression proteomics – methodology, methods (2D HPLC, 2D PAGE, 2D imaging, MS: bottom-up, top-down)
  • 6) functional proteomics - methodology, methods (TAP-tagging, Two-Hybrid, SEAM strategy, ion-mobility strategy)
  • 7) bioinformatics in MS-assisted proteomics – identification of protein in organisms with and without sequenced genoms (MS BLAST, MultiTag)
  • 8) quantitative analysis of protein complexes – internal standard method; mass tagging (ICAT, ICMT, TMT, MCAT, GIST, SILAC, SIL), absolute quantification of proteins and peptides by MS
  • 9) structural proteomics – methodology (chemical cross-linking), methods (FTICR MS)
  • 10) lipidomics (analysis of lipids and cellular membranes) – fundamentals (structure and function of cellular membranes and their components), analytical approaches: lipid extraction, TLC, GC, LC, SFC, GPC, MS analysis (PIS, HGS, FAS), shot-gun analysis
Assessment methods (in Czech)
Přednáška je založena na prezentaci, která bude k dispozici ve formátu PDF. Předmět je ukončen ústní zkouškou.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008.
  • Enrolment Statistics (Autumn 2006, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2006/C7940