PřF:C8857 Study of interactions between

C8857 Study of interactions between proteins and DNA

Faculty of Science
Autumn 2006

Extent and Intensity

1/0/0. (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Mgr. Lumír Krejčí, Ph.D. (lecturer)

Guaranteed by

Timetable

Thu 13:00–13:50 C04/211

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 15 fields of study the course is directly associated with, display

Syllabus

1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II

Language of instruction

English

Further Comments

Study Materials
The course is taught annually.