Bi7340c Cytology and morphology of bacteria - practical course

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Ludmila Tvrzová, Ph.D. (seminar tutor)
Guaranteed by
RNDr. Ludmila Tvrzová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Ludmila Tvrzová, Ph.D.
Prerequisites
B4090 General microbiology && NOW( B7330 Cytology and morphology of bacteria )
general microbiology cytology and morphology of bacteria - lecture
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
The aim of this practical laboratory course is to give the possibility to observe bacteria in their morphological diversity and to study the structures of bacterial cells. The course could give basic practical knowledge of methods of microscopic slides preparation, microscopy and image acquisition.
Syllabus
  • Morphology of bacterial cells and colonies, basic staining methods. Techniques of work with light microscope, care, removing of simple problems. Microphotography, digital photography, the use of video-cameras in image acquisition in microbiology, computer image analysis. Determination of the size of bacterial cells and their morphological structures(the use of ocular micrometer and software for image analysis). Gram staining. Acid-fast bacteria, Ziehl-Neelsen staining, mycobacteria. Giemsa staining. Endospores and sporulation of bacteria. Phase contrast and Nomarski diferential interference contrast. Capsules, sheaths and slimes of bacterial cells. Movement of bacterial cells. Technique of slide cultures. Actinomycetes and methods of their morphology observation. Measurment and comparing of proportions of selected groups of microorganisms. Staining of cell wall, nuclear material and reserved compounds in cells of bacteria and yeasts.
Literature
  • PERRY, Jerome J. and James T. STALEY. Microbiology :dynamics and diversity. Fort Worth: Saunders College Publishing, 1997, xxxi, 911. ISBN 0-03-053893-9. info
  • Laboratory experiments in microbiology. Edited by Ted R. Johnson - Christine L. Case. 4th ed. Redwood City: Benjamin Cummings, 1995, xi, 418 s. ISBN 0-8053-8509-6. info
Assessment methods (in Czech)
praktická cvičení zápočet
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014.