PřF:Bi9310 Real-time PCR - Course Information

Bi9310 Real-time polymerase chain reaction

Extent and Intensity

2/2. 4 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

doc. RNDr. Petr Vaňhara, Ph.D. (lecturer)
Mgr. Zdeněk Ručka, Ph.D. (seminar tutor)
doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Vaňhara, Ph.D.

Timetable of Seminar Groups

Bi9310/01: No timetable has been entered into IS. Z. Ručka, S. Ševčíková, P. Vaňhara
Bi9310/02: No timetable has been entered into IS. Z. Ručka, S. Ševčíková, P. Vaňhara
Bi9310/03: No timetable has been entered into IS. Z. Ručka, S. Ševčíková, P. Vaňhara

Prerequisites (in Czech)

Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20

fields of study / plans the course is directly associated with

• Cellular and Molecular Diagnostics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

At the end of this course, students should be able to:
understand PCR and real-time PCR principles
design a real-time PCR experiment, including template manipulation, chemistry and instrumentation
evaluate absolute and relative quantification data, SNP analysis and alelic discrimination and high resolution melting analysis

Syllabus

• PCR and real-time PCR principles
• Semiquantitative and quantitative PCR
• PCR kinetics, end point vs. real-time PCR
• Absolute and relative quantification, PCR efficiency
• Template manipulation and quality, storage
• Reverse transcription
• Principles of fluorescence emmission and detection
• Reporter fluorophores, quenchers, probe structure
• Primer and probe design
• General applications of real-time PCR
• Experimental design, controls, reference genes, good laboratory practice
• Data interpretation: absolute and relative quantification, SNP, high resolution melting analysis
• Factors influencing PCR, normalisation, design of student's own experiments

Literature

• Real-time PCR. Edited by M. Tevfik Dorak. New York: Taylor & Francis, 2006, xxvi, 333. ISBN 041537734X. info
• A-Z of quantitative PCR. Edited by Stephen A. Bustin. La Jolla, Calif.: International University Line, 2004, xxix, 882. ISBN 0963681788. info

Teaching methods

lectures, practical training

Assessment methods

Lectures, laboratory training
Final test

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on the extent and intensity of the course: v týdenním bloku.

• Enrolment Statistics (Autumn 2009, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2009/Bi9310