Bi9310 Real-time polymerase chain reaction

Faculty of Science
Autumn 2009
Extent and Intensity
2/2. 4 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Petr Vaňhara, Ph.D. (lecturer)
Mgr. Zdeněk Ručka, Ph.D. (seminar tutor)
doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Vaňhara, Ph.D.
Timetable of Seminar Groups
Bi9310/01: No timetable has been entered into IS. Z. Ručka, S. Ševčíková, P. Vaňhara
Bi9310/02: No timetable has been entered into IS. Z. Ručka, S. Ševčíková, P. Vaňhara
Bi9310/03: No timetable has been entered into IS. Z. Ručka, S. Ševčíková, P. Vaňhara
Prerequisites (in Czech)
Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to:
understand PCR and real-time PCR principles
design a real-time PCR experiment, including template manipulation, chemistry and instrumentation
evaluate absolute and relative quantification data, SNP analysis and alelic discrimination and high resolution melting analysis
Syllabus
  • PCR and real-time PCR principles
  • Semiquantitative and quantitative PCR
  • PCR kinetics, end point vs. real-time PCR
  • Absolute and relative quantification, PCR efficiency
  • Template manipulation and quality, storage
  • Reverse transcription
  • Principles of fluorescence emmission and detection
  • Reporter fluorophores, quenchers, probe structure
  • Primer and probe design
  • General applications of real-time PCR
  • Experimental design, controls, reference genes, good laboratory practice
  • Data interpretation: absolute and relative quantification, SNP, high resolution melting analysis
  • Factors influencing PCR, normalisation, design of student's own experiments
Literature
  • Real-time PCR. Edited by M. Tevfik Dorak. New York, N.Y.: Taylor & Francis, 2006, xxvi, 333. ISBN 041537734X. URL info
  • A-Z of quantitative PCR. Edited by Stephen A. Bustin. La Jolla, Calif.: International University Line, 2004, xxix, 882. ISBN 0963681788. info
Teaching methods
lectures, practical training
Assessment methods
Lectures, laboratory training
Final test
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: v týdenním bloku.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (Autumn 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2009/Bi9310