C7176 DNA diagnostics

Faculty of Science
Autumn 2009
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Omar Šerý, Ph.D. (lecturer), doc. Mgr. Jan Lochman, Ph.D. (deputy)
Mgr. Lukáš Častulík (seminar tutor)
Guaranteed by
prof. RNDr. Omar Šerý, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C7176/01: Mon 16:00–19:50 C05/108, L. Častulík
C7176/02: Mon 12:00–15:50 C05/108, L. Častulík
Prerequisites
C7175 DNA diagnostics
It is necessary to absolve course DNA diagnostics.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 32 student(s).
Current registration and enrolment status: enrolled: 0/32, only registered: 0/32, only registered with preference (fields directly associated with the programme): 0/32
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to perform the practical DNA analysis - isolate DNA by different methods from different materials, use different PCR methods, restriction analysis, electrophoresis on agarose gel, DNA sequencing, and usa of immunochromatographic tests.
Syllabus
  • 1. Theory, safety of work in laboratory, work with instruments 2. The comparison of DNA isolation methods – silica columns 3. The comparison of DNA isolation methods – paramagnetic particles 4. The comparison of DNA isolation methods – phenol-chloroform 5. The comparison of conventional PCR method with nested PCR method 6. Restriction analysis and electrophoresis 7. Theory and elaboration of obtained results 8. DNA sequencing a RealTime PCR 9. Analysis of drug metabolites and other substances by rapid tests
Literature
  • Manual of commercial methods in clinical microbiology. Edited by Allan L. Truant. Washington: ASM Press, 2002, xix, 481 s. ISBN 1-55581-189-2. info
  • The nucleic acid protocols handbook. Edited by Ralph Rapley. Totowa, N.J.: Humana Press, 1999, xxii, 1050. ISBN 0896038416. info
Teaching methods
2-3 lectures with theoretical preparation and laboratory work.
Assessment methods
Graded credit according obtained laboratory protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Nutností zápisu do předmětu je úspěšné absolvování zkoušky z DNA diagnostiky.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2009/C7176