Bi7941 Molecular biology - practice

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
( Ex_3148 Obecná a anorganická chemie || Ex_3155 General Chemistry || Ex_2145 Anorganická chemie - cvičení || Ex_3148 Obecná a anorganická chemie || C1080 General Chemistry-lab.course || C1620 General and Inorg.Chem.-lab. || C1650 Fundamentals of Chem.-lab. || C2100 Inorganic Chemistry-lab.course || C2500 General Chemistry-lab.course || C2740 Fundament. of Chem.-lab.course || C2480 Fundamentals of Organic Chemistry and Biochemistry || SOUHLAS) && ( Bi4020 Molecular biology || NOW( Bi4020 Molecular biology ))
Basic knowledge of general genetics, microbiology, anorganic chemistry, organic chemistry and biochemistry.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
This practical course is conceived as an introduction into methods of molecular biology.
Syllabus
  • 1. Preparation of solutions and stock enzymes used in molecular biology.
  • 2. Isolation of plasmid DNA (pUC family, pBluescript). Determination of DNA concentration and purity.
  • 3. Restriction analysis of nucleic acids, agarose gel electrophoresis.
  • 4. Restriction mapping, isolation of DNA from agarose gels.
  • 5. DNA cloning in basic types of vectors, DNA transfer to prokaryotic cells, transformation, selection of clones.
  • 6. Centrifugation techniques, preparation of sacharose density gradients.
  • 7. Isolation of bacterial genomic DNA for DNA fingerprinting.
  • 8. Polymerase chain reaction and their modifications (PCR-RFLP, AP-PCR).
Literature
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The practical course is taught in seven 6-hour blocks in a molecular biology laboratory. Students perform the methods according to examined protocols.
Assessment methods
The course is completed after work out an elaborate from the performed tasks and pass a writen test that contained 20 practically oriented questions.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.