Bi9080 Technical processes in biotechnology

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Prerequisites
The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
Syllabus
  • Stechiometry of microbial growth. Material balances.
  • Fluids properties. Fluid transfer in piping.
  • Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
  • Influence of solvents on enzyme activities.
  • Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
  • Precipitation. Salting out. Denaturation and renaturation of proteins.
  • Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
  • . Membrane separation processes. Large scale chromatographic purification of proteins.
  • Drying. Storage and stability of proteins. Validation of production quality.
Literature
  • 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
Teaching methods
The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
Assessment methods
The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.