Bi9310c Real-time polymerase chain reaction - practice

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Mgr. Zdeněk Ručka, Ph.D. (assistant)
doc. RNDr. Sabina Ševčíková, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Vaňhara, Ph.D.
Prerequisites (in Czech)
( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data
Syllabus
  • Absolute (AQ) and relative (RQ) quantification
  • Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
  • Primary data analysis, outputs, statistics
  • MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
Literature
  • Real-time PCR. Edited by M. Tevfik Dorak. New York: Taylor & Francis, 2006, xxvi, 333. ISBN 041537734X. info
  • A-Z of quantitative PCR. Edited by Stephen A. Bustin. La Jolla, Calif.: International University Line, 2004, xxix, 882. ISBN 0963681788. info
Teaching methods
practical training
Assessment methods
active participation, protocol
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
The course is also listed under the following terms Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.