IB023 Binding and Kinetics for Experimental Biologists

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Petr Kuzmic, Ph.D. (lecturer), doc. Mgr. Ctirad Hofr, Ph.D. (deputy)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30
Syllabus
  • LECTURE TOPICS 1. Principles of data analysis: Matching models to experimental data 2. Confidence intervals for rate and equilibrium constants 3. Time course of biochemical and biophysical processes: Mathematical models 4. Experimental methods for the determination of rate constants 5. Biochemical and biophysical equilibria: Mathematical models 6. Analysis of equilibrium binding data 7. Enzyme catalysis and inhibition of enzymes 8. Choosing the appropriate molecular mechanism based on available data Notes: • All lectures will include concrete examples from published literature EXERCISES 1. Determination of a equilibrium binding constant for a protein-protein interaction 2. Determination of rate constants for protein-DNA interaction 3. Determination of stoichiometry protein-DNA interaction 4. Selecting an appropriate mechanism for enzyme inhibition Notes: • Practical sessions will be held in the computer laboratory using the software DynaFit • All exercises will include actual data published in the literature • Students are encouraged to bring their own additional data
Language of instruction
English
Further comments (probably available only in Czech)
The course is taught only once.
The course is taught: in blocks.
Note related to how often the course is taught: Výuka probíhá blokově 22.-26.11.2010.
General note: http://www.biokin.com/dynafit/index.html.
Information on the extent and intensity of the course: 1D.
The course is also listed under the following terms Autumn 2010, Autumn 2011 - acreditation.