Bi7050c Protein characterisation using mass spectrometry - practice

Faculty of Science
Autumn 2011
Extent and Intensity
0/1/0. 1 credit(s) (plus extra credits for completion). Recommended Type of Completion: z (credit). Other types of completion: graded credit.
Teacher(s)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Timetable
Fri 8:00–9:50 C02/211
Prerequisites (in Czech)
NOW( Bi7050 Mass spectrometry of proteins )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to: understand basics of protein identification based on MS a MS/MS data;
select and use correct search software for identification of proteins;
understand basics of interpretation of MS/MS spectra;
Syllabus
  • Interpretation of MS data;
  • Interpretation of MALDI-MS/MS and ESI-MS/MS data;
  • Protein identification based on MS and MS/MS data;
  • Introduction into de novo sequencing;
  • Characterisation of protein modification - determination of type and site;
Literature
    recommended literature
  • SNYDER, Peter A. Interpreting protein mass spectra :a comprehensive resource. Oxford: Oxford University Press, 2000, xi, 522 s. ISBN 0-8412-3571-6. info
Teaching methods
practical work with data using PC
Assessment methods
individual processing of test data
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012.
  • Enrolment Statistics (Autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2011/Bi7050c