Bi7590 Mammalian transgenosis

Faculty of Science
Autumn 2011
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Aleš Knoll, PhD. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Aleš Knoll, PhD.
Prerequisites (in Czech)
Bi6400 Methods of molecular biology
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
At the end of the course students should be able to: understand the current state of knowledge in the field of transgenic mammals, explain the importance of transgenic mammals, understand the transgenesis techniques and interpret the implications and potential problems of creating of transgenic mammals.
Syllabus
  • The importance and prospects of transgenic mammals (growth, resistance, quality, biofermenters, xenotransplantation, etc.)
  • Recombinant DNA technology
  • Gene constructs (mammalian expression vectors)
  • Creation of genetically modified somatic cells (gene therapy)
  • Creation of genetically modified germinal cells (germinal transgenesis)
  • Techniques of gene transfer (in gametes, embryos and somatic cells)
  • The relationship between transgenesis and cloning of mammals Model organisms: transgenic mouse and gene knock-out
  • Examples of transgenic farm animals
  • Legal and ethical aspects of cloning and transgenesis, discussion
Literature
    recommended literature
  • Transgenesis Techniques: Principles and Protocols (Methods in Molecular Biology). Elizabeth J. Cartwright (Ed.). Humana Press; 3rd ed., 2009, 348 s. ISBN 978-1-60327-018-2
  • HOUDEBINE, Louis-Marie. Animal transgenesis and cloning. [Chichester]: Wiley, 2003, xiv, 220. ISBN 0470848286. info
Teaching methods
lectures, class discussion
Assessment methods
oral exam
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2013, Autumn 2014.
  • Enrolment Statistics (Autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2011/Bi7590