PřF:Bi7120 Molecular biology of prokaryot - Course Information
Bi7120 Molecular biology of prokaryotes
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Jiří Doškař, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 11:00–12:50 B11/306
- Prerequisites
- ( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS )
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to understand how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizotntal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
- Syllabus
- Terminology of bacterial genetics
- Structure and reproduction of bacterial chromosome
- Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
- F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
- Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
- Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
- Transformation
- Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
- Restriction and modification of DNA, RM systems of bacteria
- Molecular evolution of prokaryotic genomes.
- Literature
- LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
- Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
- SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
- JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
- Teaching methods
- Lectures are based on power-point pictures, tables and texts elaborated accordindg to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
- Assessment methods
- Visiting of lectures is not obligatory. Oral examination follows thewritten test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- http://orion.sci.muni.cz/kgmb/Student/7120.htm
- Enrolment Statistics (Autumn 2012, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2012/Bi7120