Bi9410 Structural Biology

Faculty of Science
Autumn 2012
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Jan Brezovský, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. Mgr. Jiří Damborský, Dr.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 12:00–13:50 B11/235
Prerequisites
Knowledge on the level of basic lectures of biochemistry or molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to introduce the biological systems from the point of view of biomacromolecular structures.
Students will get an overview of methods for determination, retrieval and visualisation of structures, and learn how to infer useful information about their function. Students will also learn basics of important biological phenomena such as protein dynamics or protein-protein, protein-DNA and protein-ligand interactions. At the end of the course, an application of structural biology to the study of molecular basis of essential biological processes, drug design and engineering of novel biocatalysts will be introduced.
Syllabus
  • Structural biology is a scientific discipline derived from molecular biology, biochemistry and biophysics. It focuses on the molecular structure of biomacromolecules, especially proteins and nucleic acids which are essential components of all living organisms. Structural biology enables us to determine a structure of biomacromolecules and to investigate its relationship to the biological role of the molecules.
  • Within this course, the following topic will be addressed:
  • 1. Structure of biomacromolecules – composition, methods for determination, application in biology, visualization.
  • 2. Databases of experimental structures – deposition, retrieval and evaluation of macromolecular structures; selected structural databases.
  • 3. Models of structures – databases of models, methods for model quality evaluation, preparation of models.
  • 4. Stability and dynamics of macromolecules – analysis of molecular dynamics and stability; databases.
  • 5. Analysis of protein structure – identification of important regions: binding/active sites, transport pathways, flexible regions, binding/catalytic amino acids.
  • 6. Prediction of structure of macromolecular complexes.
  • 7. Protein-protein complexes – evaluation of complex, analysis of interacions; databases.
  • 8. Protein-DNA complexes – evaluation of complex, analysis of interacions; databases.
  • 9. Protein-ligand complexes – evaluation of complex, analysis of interacions; databases.
  • 10. Modification of protein structure – evaluation of the effect of mutation on protein structure and function.
  • 11. Application of structural biology – biological research, drug design and development, design of biocatalyst.
Literature
  • Computational Structural Biology: Methods and Applications, T. Schwede & M. C. Peitsch, World Scientific Publishing Company, 2008
  • Protein Stucture and Function, G. A. Petsko & D. Ringe, New Science Press, 2004
  • Structural Bioinformatics, J. Gu &P. E. Bourne, Wiley-Blackwell, 2009
  • Textbook Of Structural Biology, A. Liljas, L. Liljas, J. Piskur, G. Lindblom, P. Nissen, M. Kjeldgaard, World Scientific Publishing Company, 2009
Teaching methods
lectures
Assessment methods
written test
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2012, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2012/Bi9410