PřF:Bi9901 Free radicals in anim. phys. - Course Information

Bi9901 Free radicals in animal physiology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Milan Číž, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Antonín Lojek, CSc. (lecturer)

Guaranteed by

doc. RNDr. Milan Číž, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Antonín Lojek, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Tue 14:00–15:50 BFU

Prerequisites (in Czech)

Bi3030 Animal physiology && ( Bi5220 Immunology || Bi6930 Immunotoxicology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)

Course objectives

At the end of the course students should be able to:
understand molecular principles of origin and activity of reactive oxygen and nitrogen species;
explain their physiological role in animal organism;
review the role of free radicals in diseases;
categorize defence reactions against free radicals;
understand principles of methods evaluating oxidative damage.

Syllabus

• Biology of oxygen (Oxygen and evolution of life on Earth; Oxygen cycle in nature; Photosynthesis; Respiratory chain). Chemistry of free radicals (Reactive oxygen species; Role of transition metal ions; Nitric oxide and derived molecules). Sources of free radicals (Endogenous physiological or pathological processes: Leak of electrons from metabolic pathways, Peroxisomes, Xanthine/xanthine oxidase, Metabolism of arachidonic acid, Autooxidation of catechols and flavonoids; Exogenous sources, Food constituents, Drugs, UV irradiation, Ionising irradiation, Polluted environment). Physiology of free radicals (Phagocytosis; NADPH oxidase of professional phagocytes; Microbicidal mechanisms based on oxygen; Chronic granulomatous disease (CGD); Gene regulation; Apoptosis; Oxidative burst in plants as defence mechanism against infection). Role of free radicals in diseases (Lipid peroxidation; Protein degradation; DNA damage; Regulation of oxidative stress). Defence against free radicals (Prevention of free radical generation; Compartmentalisation of free radicals; Free radical scavengers; Antioxidant defence and damage repair; Antioxidant pharmacological intervention). Pathology of free radicals (Oxidative stress and aging; Reproduction; Ischemia-reperfusion injury; Inflammation; Atherosclerosis; Brain dysfunction; Cancer; Myocarditis; Diabetes mellitus; Eye diseases; Adult respiratory distress syndrome (ARDS); Oxidative injury to lungs induced by xenobiotics; Radiation injury; Rheumatoid arthritis; Skin injury). Methods of oxidative damage evaluation (Detection of free radicals; Evaluation of antioxidant activity of biological samples; Determination of damage to biological macromolecules).

Literature

• ŠÍPEK, Stanislav. Antioxidanty a volné radikály ve zdraví a v nemoci. 1. vyd. Praha: Grada, 2000, 314 s. ISBN 8071697044. info

Teaching methods

Lectures and presentations by specialists in the individual sectors of the field. Reading of scientific articles, class discussion.

Assessment methods

Written examination which consists of 12 questions. It is necessary to obtain at least 16 points (maximum 30 points) for succesful passing out.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught once in two years.
Information on course enrolment limitations: Min. počet zapsatelných studentů: 5. Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!

• Enrolment Statistics (Autumn 2012, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2012/Bi9901