PřF:Bi7312 Eukaryotic cell in practice - Course Information

Bi7312 Practical course of molecular biology of eukaryotes

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. Mgr. Petr Beneš, Ph.D. (seminar tutor)
Mgr. Lucia Knopfová, Ph.D. (seminar tutor)
Mgr. Jarmila Navrátilová, Ph.D. (seminar tutor)
prof. RNDr. Jan Šmarda, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7312/01: No timetable has been entered into IS. P. Beneš
Bi7312/02: No timetable has been entered into IS. P. Beneš
Bi7312/03: No timetable has been entered into IS. P. Beneš
Bi7312/04: No timetable has been entered into IS. P. Beneš

Prerequisites

NOW( Bi7090 Eukaryotic cells )
The course requires basic practical skills of microbiology, biochemistry and molecular biology as, for example, preparation of buffers, cultivation media, sterile liquide handling, inoculation, centrifugation and so on.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 25 student(s).
Current registration and enrolment status: enrolled: 0/25, only registered: 0/25

fields of study / plans the course is directly associated with

• Molecular Biology and Genetics (programme PřF, N-BI)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB, specialization Antropogenetika)

Course objectives

At the end of the course students acquire practical skills with basic techniques allowing manipulations with animal and human cells in vitro. They will understand how to perform genetic modification of cells in culture, how to analyze cell morphology, proliferation, differentiation, migration and apoptosis.

Syllabus

• 1. Splitting of suspension and adherent mammalian cells. 2. Transient transfection of mammalian cells with the plasmid cmvGFP by electroporation. Analysis of transfection efficiency by fluorescence microscopy and flow-cytometry. 3. Analysis of activity of transcription factor RAR (transient transfection, assays of beta-galactosidase and luciferase activities). 4. Study of cellular morphology, protein expression, protein localisation in various phases of cellular differentiation (cytocentrifugation, fixation, light microscopy, fluorescence microscopy, cell fractionation, protein electrophoresis and immunoblotting). 5. Analysis of cellular markers of apoptosis (staining of viable cells by eosin dye exclusion, analysis of nuclear morphology, PARP cleavage, phosphatidylserine externalization). 6. Determination of cell proliferation and migration (cell counting, crystal violet staining, cell cycle analysis, real-time cell proliferation and migration monitoring).

Literature

• GREEN, Michael R. and Joseph SAMBROOK. Molecular cloning : a laboratory manual. 4th ed. New York [N.Y.]: Cold Spring Harbor Laboratory Press, 2012, xxxiii, s. ISBN 9781936113422. info

Teaching methods

brief lectures followed by practical training, preparation of final group project, protocols describing the experiments and summarizing the results obtained are required

Assessment methods

Credits are given for active work at a bench, participation i each lesson, preparation of final group project, elaboration of detailed protocols describing the way of performing experiments and discussion of results and conclusions obtained.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

• Enrolment Statistics (Autumn 2014, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2014/Bi7312