Bi7016 Nucleic acids chemistry - lab. practice

Faculty of Science
autumn 2017
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
Ing. Zuzana Ferenčíková, Ph.D. (seminar tutor)
doc. RNDr. Miroslav Fojta, CSc. (seminar tutor)
prof. Mgr. Václav Brázda, Ph.D. (assistant)
Mgr. Marie Brázdová, Ph.D. (assistant)
Mgr. Hana Pivoňková, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Fojta, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi7015 Nucleic acids chemistry )
Previous or parallel passing-out the Bi7015 subject
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will be trained to perform simple experiments based on methods and experimental techniques that are related to topics of the course Bi7015.
Syllabus
  • 1. Gel electrophoresis of nucleic acids, proteins and their complexes, two-dimensional electrophoresis. 2. Blotting, protein immunodetection. 3. Magnetic separation techniques, immunoprecipitation. 4. Centrifugation methods, DNA precipitation, extraction with organic solvents, gradient centrifugation, ultrafiltration. 5. Chromatographic methods, FPLC of proteins on ionex, affinity and gel-filtration columns. 7. DNA sequencing and footprinting. 7. Electrochemical methods, determination of DNA and proteins, detection of DNA damage and interactions.
Literature
  • BLACKBURN, M. G. and M. J. GAIT. Nucleic Acids in Chemistry and Biology. Oxford: Oxford University Press, 1996. info
  • SINDEN, Richard R. DNA structure and function. San Diego: Academic Press, 1994, xxiii, 398. ISBN 0126457506. info
  • ADAMS, R. L. P., J. T. KNOWLER and D. P. LEADER. The Biochemistry of Nucleic Acids. 10th edit. London: Chapman and Hall, 1986. info
Teaching methods
Practical courses, demonstrations in the laboratory
Assessment methods
Teaching method: practical demonstrations of instruments and real experimental data, simple experiments conducted by the students Type of exam: credit (given on the basis of participance)
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020.
  • Enrolment Statistics (autumn 2017, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2017/Bi7016