Bi7330 Cytology and morphology of bacteria

Faculty of Science
autumn 2017
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Gabriela Rotková, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Gabriela Rotková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Thu 14:00–15:50 E25/209
Prerequisites
general microbiology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students will understand the problematics of cytology and morphology of bacterial cells and the techniques of microscopic and macroscopic assessments of bacterias. Students will be able to characterize cytological structure, their functions and stages in more or less complex life-cycles. Student will be also able to consider the formation of the bacterial biofilm and its importance and signal processes and interactions between bacterial cells. For a specific purpose, students will be able to consider the suitability and applicability of particular methods - microscopy, archiving of acquired material, processing and analysis of images. Abilities from that field will be useful not only in biological sciences, but will also help to understand the physiological processes of bacterial cells.
Syllabus
  • Microscopic techniques, light, fluorescent and electron microscopy. Phase contrast and Nomarski contrast.
  • Microphotography, digital photography, video and digital cameras, image acquisition.
  • Image processing and analysis software, imaging techniques.
  • The structural concept of the bacterial cell. Determination of cell shape. The cytosol. Cell wall of bacteria. Structural and biochemical features of cell walls. Teichoic and teichuronic acids. Lipids and waxes, proteins. Polysaccharides. Peptidoglycan: Molecular composition, structure, and implications for taxonomy. The outer membrane. Acidoresistant bacteria. Capsules, sheets and slimes of bacteria. Inclusion bodies. Bacterial ribosome, nucleoid, cytoplasmatic membrane. Ultrastructural aspects.
  • Modelling of growth patterns in bacterial colonies
  • Structural and functional aspects of bacterial motility. Flagella. Pili and fimbriae.
  • Growth cycles of bacteria.
  • Resting stages of bacteria. Endospores, exospores, cysts.
  • Dimorphic prosthecate bacteria. Chlamydiae.
  • Complex growth cycles. Actinomycetes and myxobacteria.
  • Cyanobacteria.
  • The role of molecular composition of bacterial cell in classification and identification of bacteria.
Literature
  • PERRY, Jerome J. and James T. STALEY. Microbiology :dynamics and diversity. Fort Worth: Saunders College Publishing, 1997, xxxi, 911. ISBN 0-03-053893-9. info
Teaching methods
theoretical preparing for laboratory work in practical course of Cytology and Morphology of bacteria using illustrative schemes and image documentation
Assessment methods
recommended lectures, training in writing abstracts, oral colloquium
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
http://www.sci.muni.cz/mikrob/cytologieosn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2025.
  • Enrolment Statistics (autumn 2017, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2017/Bi7330