C5720 Biochemistry

Faculty of Science
autumn 2017
Extent and Intensity
4/0/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Oldřich Janiczek, CSc. (lecturer)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Oldřich Janiczek, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Mon 14:00–15:50 C05/114, Tue 14:00–15:50 C05/107
Prerequisites
!NOWANY( C3580 Biochemistry )
Organic Chemistry I, II or Backgrounds of Organic Chemistry. Physical Chemistry I, II or Backgrounds of Physical Chemistry.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand and explain - Structure of Living Matter. Composition and Organization of Cells. Basic Classes of Biomolecules: Amino Acids and Proteins, Sugars and Polysaccharides, Nucleic Acids, Lipids and Membranes. General Features of Metabolism. Energetics of Biochemical Reactions. Introduction to Enzymes and Coenzymes. Metabolic Fate of Biomolecules. Metabolism of Amino Acids and Proteins. Metabolism of Nucleic Acids and Proteosynthesis. Metabolism of Saccharides and Lipids. Basic Bioenergetic Metabolism. Integration of Metabolism, Regulation Principles. Membrane Transport. Special Biosynthetic Pathways.
Syllabus
  • 1. Introduction. History, objects and methods of study. Structural levels. Practical aspects. Amino acids. Structural features and properties. Methods of study.
  • 2. Peptides. Structure and nomenclature. Analysis and synthesis. Important peptides.
  • 3. Proteins. Structure and properties. Classification. Conformation and its changes.
  • 4. Methods of isolation and study of proteins.
  • 5. Function of proteins - catalytic, structural, transport, signal, defense.
  • 6. Nucleic acids. Structure and metabolism of polynucleotides.
  • 7. Saccharides. General properties and reactions of monosaccharides.
  • 8. Di-, oligo- and polysaccharides.
  • 9. Lipids. Composition and properties, classification, function. Structure of biomembranes.
  • 10. General features of metabolism, anabolic and catabolic processes. Chemical reactions, equilibrium, energetics. Macroergic compounds.
  • 11. Enzymes. Conditions of enzymatic reactions. Enzyme classification and nomenclature. Measurement of activities, units. Isolation of enzymes.
  • 12. Enzyme kinetics. Organization of enzymes, regulation.
  • 13. Coenzymes, function, classification. Typical examples.
  • Enzyme classification and nomenclature. Measurement of activities, units. Isolation of enzymes.
  • 14. Interrelationships in metabolism. Coupled reactions. Macroergic compounds, properties, function, typical representatives.
  • 15. Proteins catabolism. Digestion. General conversion of amino acids. Nitrogen metabolism.
  • 16. Amino acids metabolism, interconversion and catabolism.
  • 17. Degradation and synthesis of nucleic acids. Nucleotide metabolism, synthesis and degradation.
  • 18. Genetic information, its transfer and expression. Protein synthesis, regulatory mechanisms.
  • 19. Metabolism of saccharides. Degradation and biosynthesis of polysaccharides.
  • 20. Glycolysis. Reaction scheme, energetics, regulation. Resynthesis of glucose. Other conversions of saccharides. Hexose monophosphate pathway, pentose cycle.
  • 21. Metabolism of lipids. Degradation and biosynthesis of fats and phosphatides. Degradation and biosynthesis of fatty acids. Ketonic compounds.
  • 22. Tricarboxylic acids cycle. Reaction scheme, energetics. Metabolic relations, regulation.
  • 23. Biological oxidation. Types and role, examples. Energetics. Oxidative phosphorylation. The organization of respiratory chain. Mechanism of electron transport, generation and use of protonmotive force, ATP synthesis.
  • 24. Photosynthesis. Pigments, organization, electron transport. CO2 fixation.
  • 25. Microsomal electron transport, nitrogenase system. Oxygenation.
  • 26. Porphyrins. Heme biosynthesis and degradation. Isoprenoids. Biosynthesis. Terpenoids, carotenoids, vitamins, isoprenic quinones. Steroids, nomenclature, biosynthesis, conversions. Hormones, bile acids, vitamin D. Biosynthesis of aromatic compounds. Alkaloids, basic principles of their biosynthesis.
  • 27. Regulation of metabolism. Mutual relations in metabolism. Regulatory levels. Regulation of enzyme activity, metabolic pools. Compartmentation. Membrane gradients, solute transport. Types,energetics. Important translocase systems.
  • 28. Neurohumoral system. Mechanism of hormone action. Mineral metabolism, the role of ions in biochemical processes. Nerve cell excitation, role of ions and neurotransmitters.
Literature
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • DUCHOŇ, Jiří. Lekárska chémia a biochémia. 1988, 749 s. info
  • DUCHOŇ, Jiří. Přehled lékařské chemie a biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1982, 306 s. info
  • DUCHOŇ, Jiří. Přehled lékařské chemie a biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1982, 666 s. info
  • DUCHOŇ, Jiří. Přehled lékařské chemie a biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1982, 306 s. info
Teaching methods
Theoretical lecture.
Assessment methods
Lecture. Oral examination.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020.
  • Enrolment Statistics (autumn 2017, recent)
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