Bi8011c Microbial zoonoses and sapronoses - practice

Faculty of Science
Autumn 2019
Extent and Intensity
0/2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Ivo Rudolf, Ph.D. (seminar tutor)
Mgr. Silvie Šikutová, Ph.D. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Ivo Rudolf, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
NOW( Bi8011 Microbial zoonoses sapronoses )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, the students should be well informed about the selected methods of collection of haematophagous arthropods, they should be able to work safely in laboratories with zoonotic microorganisms, to prepare cell cultures, and to use basic molecular methods apllicable for detection of microorganisms in haematophagous arthropods (PCR, RT-PCR, real-time PCR).
Learning outcomes
Output will be determination of vector species and results of carried out molecular analyses of presence microbial pathogens in sampled vectors.
Syllabus
  • 1. Demonstration of collection techniques for selected haematophagous arthropods (especially ixodid ticks and mosquitoes) in the field; determination of arthropods in the laboratory. 2. Examination of microscope slides and colour figures of zoonotic pathogens. 3. Guidelines for safe work in the laboratory handling zoonotic microorganisms - safety rules in infectious environment (the work in biohazard and laminar boxes, material decontamination). 4. Serological methods – detection of antibodies to selected vertebrate pathogenic viruses (PRNT, ELISA): demonstration of the tests. 5. Basics of the work with cell cultures: practical steps - preparation of the cultures, their infection with a virus, and inspection for cytopathic effect. 6. Molecular techniques used for the detection of microorganisms in haematophagous arthropods (PCR, RT-PCR, real-time PCR), demonstrations - isolation of DNA and RNA; PCR; gel electrophoresis, photodocumentation of the gel, purification of PCR products).
Literature
    recommended literature
  • HUBÁLEK, Zdeněk and Ivo RUDOLF. Mikrobiální zoonózy a sapronózy (Microbial zoonoses and sapronoses). 2., přepracované a doplněné. Brno: Masarykova univerzita, 2007, 176 pp. ISBN 978-80-210-4460-9. info
Teaching methods
Theoretical instructions, demonstrations, field trip, and laboratory work.
Assessment methods
Discussions with students (feedback).
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every other week.
General note: Předmět se doporučuje zapsat v 1. nebo 3. semestru.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2019, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2019/Bi8011c