Bi5130c Introduction to human aDNA studies practical training

Faculty of Science
Autumn 2020
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. et Mgr. Kristýna Brzobohatá, Ph.D. (seminar tutor)
Guaranteed by
Mgr. et Mgr. Kristýna Brzobohatá, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. et Mgr. Kristýna Brzobohatá, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the course is practical skills of genetic analysis of human ancient DNA - selection of a suitable sample, treatment and purification of selected tissue, DNA isolation, DNA amplification, DNA sequencing and evaluation of results. Students will also learn to work under DNA-free conditions.
Learning outcomes
After course, the student will be able to: - select a suitable part of human skeleton for analysis of aDNA and separate methods by appropriate methods - clean and treat the selected tissue to minimize the risk of recent DNA contamination - lysate bone tissue and purify aDNA - select the amplification protocol for a given target sequence (primer design, PCR modification ...) - Visualize the PCR products via ELFO and enzymatically purify them for sequencing - evaluate the acquired sequence and interpret it in historical contexts
Syllabus
  • Tissue collection, sample cleaning, bone tissue lysis Isolation of a DNA Amplification of HVR I mtDNA ELFO, sample preparation for sequencing Evaluation of results
Literature
  • HUMMEL, Susanne. Ancient DNA typing : methods, strategies and applications. Berlin: Springer, 2010, xii, 298. ISBN 9783642077050. info
Teaching methods
Laboratory practice
Assessment methods
Protocol indicating: - the material and methods used - photos of analyzed samples, determination of their preservation - a slide of ELFO PCR products, an evaluation of the success of the reaction - the electropherotram of the sequences obtained - a summary of the sequence obtained - putting into a historical context (e.g., mtDNA)
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Note related to how often the course is taught: jednou za 14 dní 4 hodiny pro skupinu studentů.
The course is also listed under the following terms autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2024.
  • Enrolment Statistics (Autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2020/Bi5130c