Bi9250 Special Immunological Methods

Faculty of Science
Autumn 2020
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Žákovská, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Lukáš Kubala, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Žákovská, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 8:00–9:50 C13/332
Prerequisites (in Czech)
Bi5220 Immunology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to: understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research; create reasoned decisions about immunological problems in diagnosis; make reasoned decisions about immunological problems in diagnosis; make deductions based on acquired knowledge of immunological methods; interpret immunological methods;
Learning outcomes
At the end of the course students should be able to: understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research; create reasoned decisions about immunological problems in diagnosis; make reasoned decisions about immunological problems in diagnosis; make deductions based on acquired knowledge of immunological methods; interpret immunological methods;
Syllabus
  • 1.-3. lecture: organization of lectures and practice course; Methods used in clinical praxis; Serological method (precipitation, immunodiffusion, immunoelectrophoretic, agglutination hemagglutination, complement methods, immunoblotting),Detection of spirochaetal infection,fecal Bacteriotherapy
  • 4. lecture: nephelometry, turbidimetry
  • immuno-chemical methods, RIA, FIA, EIA
  • 5. lecture: EIA, ELISA method in detail, different kinds of ELISA, workshop of home-made ELISY
  • 6. lecture: a) work with the immune system cell, the determination of leukocyte populations and their function b) special in vitro tests- for examination of leukocyte populations and their function, tests of lymphocyte proliferation, test of lymphocyte transformation test, investigation of HLA antigens
  • 7. lecture: Alergological examination
  • 8.lecture: Monoclonal antibodies - hybridisation method and use in practice, basic interpretation of the results of immunological lab tests, methods used in clinical labs, microarray
  • 9. lecture: 9.-13. lectures: Excursion in labs: Biovendor, Test Line, Viameda, VÚVel
Literature
  • Doporučená literatura k předmětu Speciální imunologické metody – přednáška a cvičení
  • Doporučená literatura k předmětu Speciální imunologické metody – přednáška a cvičení
Teaching methods
Theoretically formulation for management of the immunological techniques, which are used in praxis; presentations by professionals in the form of internship programme in private immunological and experimental labs.
Assessment methods
The students participation at lectures is demanded. The final clafficiation will be in the form of written test at the end of term.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2020/Bi9250