S2012 Applying Mendel’s laws of genetics to plant biology in the lab

Faculty of Science
Autumn 2020
Extent and Intensity
1/2/0. 3 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Helene Robert Boisivon, Ph.D. (lecturer)
Shekoufeh Ebrahimi Naghani, Ph.D., MSc (seminar tutor)
doc. Mgr. Jan Havliš, Dr. (assistant)
Guaranteed by
Helene Robert Boisivon, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: Helene Robert Boisivon, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Mon 9:00–9:50 E26/222, Mon 10:00–11:50 E26/222
Prerequisites
The target audience is BSc and MSc students, suitable also for starting PhD students. During the course, all necessary information will be provided. However, a basic knowledge of genetics is welcome.
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
Course objectives
This course will use various laboratory techniques to test the segregation law of genetics as established by G. Mendel, the correlation between genotype and phenotype, and the effects of plant hormones for plant development. The lectures are accompanied by practical work. In case of eLearning and distance-teaching imposed by the COVID situation, only the theorical part will be taught remotely via Teams.
Learning outcomes
Students should be able to understand the analysis of segregation for a single genetic character, and the link between genotype and phenotype. Students will learn basic lab techniques. Students will be initiated to the role of plant hormones in plant development.
Syllabus
  • Using Arabidopsis mutants the students will observe and calculate the frequencies of the mutant’s phenotypes, and correlate it with the genotype of individual seedlings. This part of the practical uses laboratory techniques such as work in sterile conditions, sow seeds for in vitro culture, monitoring seedling growth and analysis of the seedling phenotypes by stereomicroscope.
  • In a second part of the course, the student will investigate the effects of the plant hormone auxin on root growth (few concentrations tested) and on reporter lines for the hormone signalling pathway. Activation or repression of the signalling pathways will be tested by colorimetric staining. Results will be collected and conclusion will be written and discussed. This part of the practical includes tissue culture as in the first part (including preparation of the growth medium), result analysis (root growth measurements), colorimetric staining and microscopy.
Teaching methods
Lectures, exercises and practical work. Sharing information and documents will be done through Teams. Registered students will receive an invitation.
Assessment methods
Test and practical report.
Evaluation of the course is based on short exercises and practical report.
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
Planned schedule

Mon. 5/10 9.00

Theory part 1 (Role of auxin for plant development, Auxin signalling and auxin transport, pin1 and monopteros mutants)

Preparative lecture Practical 1

Fri. 9/10 9.00

Practical part 1

Mon. 12/10

Theory part 2 (Mendel’s law of genetics, Molecular genetics in Arabidopsis thaliana)

Practical 1

Mon. 19/10

Practical part 1

Mon. 26/10

Practical part 1

Mon. 2/11 9.00

Theory part 3 (Root development, Effects of auxin for root growth)

Preparative lecture Practical 2

Practical part 1

Mon. 9/11 9.00

Practical part 2

Thur. 12/11

Practical part 2

Mon. 16/11 9.00

Practical part 2

Wed. 18/11 9.00

Practical part 2

Mon. 23/11 9.00

Practical part 2

Mon. 30/11 9.00

Practical part 2

Mon. 7/12 9.00

Practical part 2

Mon. 11/1

Report handling

The course is also listed under the following terms Autumn 2019, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2020/S2012