PřF:C7955 Molecular luminescence - Course Information

C7955 Molecular luminescence

Extent and Intensity

2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

doc. Mgr. Petr Táborský, Ph.D. (lecturer)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)

Guaranteed by

doc. Mgr. Petr Táborský, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science

Timetable

Tue 11:00–12:50 C14/207

Prerequisites

Knowledge of analytical chemistry on a level of introductory course. Knowledge of high school physics.

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 2/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30

Course objectives

The course will provide information on physical principles of molecular luminescence and its applications in inorganic, organic and especially biochemical analysis. At the end of the course students also should understand to modern application of luminescence spectroscopy and related techniques such as GFP, FRET, FLIM, polarisation of fluorescence, Fluorescence in situ hybridization, etc.

Syllabus

• 1. Introduction to optical methods, history. Absorption of light by molecule, electron transitions, Franck-Condon principle. Jablonski diagram, luminescence, fluorescence, phosphorescence, delayed fluorescence, basic principles. Influence of medium, pH, polarity, etc. Luminescence quenching, dynamic and static collisions. 2. Basic units, luminescence spectra: excitation and emission spectra, Stokes shift, mirror rule. Scattering. Basic relationships, energetic and quantum yields. Luminescence lifetime. 3. Structure of compounds and luminescence, luminescence of organic molecules, transitions, general rules. Fluorescence spectra of biomolecules. Luminescence properties of inorganic compounds. Luminescence of solid phase. Interpretation of spectra: concentration, structure. Intrinsic and extrinsic luminescence. 4. Instrumentation, basic system, spectrofluorometers, excitation sources, monochromators and detectors. 5. Measurement and data treatment, 3D spectra, synchronous scan. Lifetime measurement. Fluorescence microscopy. Phosphorescence measurement. Polarisation and luminescence anisotropy. 6. Luminescence assignment: chemiluminescence, bioluminescence, electroluminescence, triboluminescence, etc. 7. Luminescence labels, derivatisation, binding sites, criterion, luminescence in clinical analysis, fluorescence imunoassays, fluor. substrates. 8. Comparison to spectrophotometry in UV-Vis region. Separation techniques with fluorescence detection, indirect determination. 9. Luminescence probes: polarity, membrane and DNA probes, sensors. 10. Fluorescence microscopy and time resolved fluorescence microscopy, flow cytometry. FISH. Fluorescence correlation spectroscopy. 11. Native luminescence of proteins and peptides, membrane modifications, Green fluorescence protein. 12. Excursion

Literature

• Principles of fluorescence spectroscopy. Edited by Joseph R. Lakowicz. 3rd ed. New York: Springer, 2006, xxvi, 954. ISBN 0387312781. info

Teaching methods

lectures

Assessment methods

Final oral examination.

Language of instruction

Czech

Follow-Up Courses

• C7940 Bioanalytics I - Biomacromolecules

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Teacher's information

http://bart.chemi.muni.cz/courses.htm

• Enrolment Statistics (autumn 2021, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2021/C7955