C9092 Bioapplications of nanoparticles

Faculty of Science
autumn 2021
Extent and Intensity
1/0. 1 credit(s) (plus extra credits for completion). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
Mgr. Antonín Hlaváček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Preparation of nanoparticles and their bioconjugates is an interdisciplinary field of research utilizing the knowledge of biochemistry, physical, analytical, organic and material chemistry with a significant impact on biological sciences. Students obtain a sufficient understanding of nanomaterial properties as well as methods necessary for their practical use. The aim of the lecture is to provide a comprehensive overview on the subject focusing on the application in biology, bioanalytical chemistry and biochemistry. The first part of the lecture describes exceptional properties of nanoparticles, their synthesis, stability and surface modification for biomolecule binding. Great attention is paid to the description of methods for preparation, purification and characterization of nanoparticles and their bioconjugates. The last part of the course describes the use of nanoparticles and their bioconjugates (nanosensors, luminescent, magnetic and other labels, smart drug delivery, biological imaging ...). Students are able to discuss the suitability of a particular type of nanoparticles, its preparation and characterization for a given biological application.
Syllabus
  • 1) Introduction, basic concepts (nanotechnology, nanoparticles, colloidal nanoparticles ...), history, overview and description of the focus of the course
  • 2) Types of nanoparticles (according to the structure, composition, properties)
  • 3) Exceptional properties of nanoparticles (magnetic, optical, catalytic properties, nanoparticles as drug carriers, nanoparticles as components of self-assembled nanostructures)
  • 4) Methods of preparation of colloidal nanoparticles (synthesis at atmospheric pressure, solvothermal synthesis, microwave and microfluidic reactors, microemulsion systems)
  • 5) methods for modification of nanoparticles surface and introduction of reactive groups (silanization, monolayer, block copolymers), stability of nanoparticles and bioconjugates
  • 6) Methods of nanoparticle bioconjugate preparation ("classic bioconjugation chemistry", chemically orthogonal methods and "click chemistry", the use of molecular biology in preparation of bioconjugates, preparation of more complicated nanostructures)
  • 7) Methods for purification of nanoparticles and nanoparticles bioconjugates (affinity, gel and ion exchange chromatography, gel electrophoresis, free flow electrophoresis, isotachophoresis, precipitation, centrifugation, dialysis, filtration and ultrafiltration, extraction)
  • 8) Methods for characterization of nanoparticles and bioconjugates (spectral methods, titration, gravimetry and TGA, calorimetry, chromatography, electrophoresis, isotachophoresis, measurement of zeta potential, dynamic light scattering, fluorescence correlation spectroscopy, nanoparticle tracking analysis, centrifugation, field flow fractionation, microscopic methods, computational methods)
  • 9) The interaction of nanoparticles with living organism
  • 10) The use of nanoparticles and their bioconjugates: bioanalytical methods utilizing nanoparticles
  • 11) The use of nanoparticles bioconjugates: targeted drug delivery, theranostics
  • 12) Using nanoparticles bioconjugates: biological imaging and nanosensors
Literature
  • HERMANSON, Greg T. Bioconjugate techniques. San Diego: Academic Press, 1996, xxv, 785. ISBN 012342335X. info
Teaching methods
lectures
Assessment methods
Student has to write answers for six questions concerning properties, preparation, characterization and application of nanoparticles which are subsequently discussed. At least two answers have to be defended successfully.
Language of instruction
Czech
Further Comments
The course is taught each semester.
The course is taught: every week.
The course is also listed under the following terms Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022.
  • Enrolment Statistics (autumn 2021, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2021/C9092