C7016 Nucleic acids chemistry - lab. practice

Faculty of Science
Autumn 2022
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Miroslav Fojta, CSc. (seminar tutor)
prof. Mgr. Václav Brázda, Ph.D. (assistant)
Mgr. Marie Brázdová, Ph.D. (assistant)
Mgr. Hana Pivoňková, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Miroslav Fojta, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Miroslav Fojta, CSc.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
NOW( C7015 Nucleic acids chemistry )
Previous or parallel passing-out the C7015 subject
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main aim of the course is to practice the knowledge gained by the students within the Bi7015 subject with an emphasis on the analytical use of the respective properties of nucleic acids.
Learning outcomes
At the end of the course the students will be able to perform • basic nucleic acids analyzes by means of spectroscopic, electrophoretic and electrochemical methods • construction of labeled oligonucleotides using DNA polymerases and labeled nucleotides • DNA cleavage by restriction endonucleases • modification of DNA using selected chemical reagents and analysis of the products
Syllabus
  • 1. Gel electrophoresis of nucleic acids, proteins and their complexes, two-dimensional electrophoresis. 2. Blotting, protein immunodetection. 3. Magnetic separation techniques, immunoprecipitation. 4. Centrifugation methods, DNA precipitation, extraction with organic solvents, gradient centrifugation, ultrafiltration. 5. Chromatographic methods, FPLC of proteins on ionex, affinity and gel-filtration columns. 7. DNA sequencing and footprinting. 7. Electrochemical methods, determination of DNA and proteins, detection of DNA damage and interactions.
Literature
  • BLACKBURN, M. G. and M. J. GAIT. Nucleic Acids in Chemistry and Biology. Oxford: Oxford University Press, 1996. info
  • SINDEN, Richard R. DNA structure and function. San Diego: Academic Press, 1994, xxiii, 398. ISBN 0126457506. info
  • ADAMS, R. L. P., J. T. KNOWLER and D. P. LEADER. The Biochemistry of Nucleic Acids. 10th edit. London: Chapman and Hall, 1986. info
Teaching methods
Practical courses, demonstrations in the laboratory
Assessment methods
Teaching method: practical demonstrations of instruments and real experimental data, simple experiments conducted by the students Type of exam: credit (given on the basis of participance)
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 1999, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/C7016