Bi9250c Special Immunological Methods - practical course

Faculty of Science
Autumn 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Lukáš Kubala, Ph.D. (seminar tutor), doc. RNDr. Alena Žákovská, Ph.D. (deputy)
Mgr. Jiří Petráš (assistant)
Guaranteed by
doc. RNDr. Alena Žákovská, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Lukáš Kubala, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi5220 Immunology && Bi5220c Immunology - pr.
Subject is connected to the basic practice course in immunology that is why students are supposed to have finished the basic practice course in immunology. The course is to extended the theory and praxis of basic immunology subject in its methods.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 14 student(s).
Current registration and enrolment status: enrolled: 3/14, only registered: 0/14, only registered with preference (fields directly associated with the programme): 0/14
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;
Learning outcomes
At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;
Syllabus
  • The practice proceeds in the form of individual parts of methods that are solved: 1. putting of antigen on the surface of microtitration plates and their preparation for ELISA test
  • 2. testing on the presence of antibodies against chosen pathogen in serum
  • 3. Test for the detection of lymphocyte activity
  • 4. Imunochromatographic tests - test for the detection of the presence of HIV, RSV antibodies in sera
  • 5. Test of indirect agglutination. Presence of erytrhrocytes Ag in saliva
  • 6. Immunocytology - invertebrate immunology, immune cells of insects
Literature
  • Bartůňková J., Paulík M. a kol.:Vyšetřovací metody v imunologii
  • Toman M., a kol. Veterinární imunologie, Grada Publishing 2000
  • Hořejší V., Bartůňková J.: Základy imunologie, Triton Praha 1998
  • Ferenčik M.: Imunochémia, 2. Vydání, Alfa Bratislava 1989
Teaching methods
laboratory exercising; using some of simple and difficult immunological methods.
Assessment methods
The students participation at lectures is mandatory. The final classification is in the form of written test at the end of term. The students are recommended to fill in written self-tests offered during the term.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2023/Bi9250c