F7810 Microscopy lab I

Faculty of Science
Autumn 2023
Extent and Intensity
0/3/0. 3 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
Mgr. Milan Ešner, Ph.D. (lecturer)
Mgr. Jana Jurmanová, Ph.D. (lecturer)
Mgr. Mojmír Meduňa, Ph.D. (lecturer)
doc. RNDr. Petr Mikulík, Ph.D. (lecturer)
Mgr. Jiří Nováček, Ph.D. (lecturer)
Mgr. Jan Přibyl, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Petr Mikulík, Ph.D.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: doc. RNDr. Petr Mikulík, Ph.D.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Prerequisites
Basic knowledge and participation in lectures related to the light and electron microscopy.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The aim of the course is to teach students how to work with electron and light microscopes, how to control them for the study of different types of samples, using different methods of contrast and signal generation, as well as to analyze the captured images.
Learning outcomes
Completing the course, the student will be able to:
- utilize various types of light microscopes using different types of contrast,
- utilize electron microscopes with various types of electron beam interaction with the sample and the signal collection,
- analyze and interpret captured images.
Syllabus
  • Light microscopy on materials (bright and dark field, interference microscopy)
  • Scanning electron microscopy (SEM), conductive materials
  • SEM on non-conductive materials, metallisation
  • Imaging and processing by ions (FIB)
  • Energy dispersive x-ray spectroscopy (EDX, WDS)
  • Optical lithography and work in clean rooms
  • Mapping the surface of samples by profilometry
  • Preparation of biological samples for light microscopy
  • Transmission light microscopy on biological samples
  • Fluorescence microscopy
  • Image data processing
Literature
  • Návody k jednotlivým úlohám
Teaching methods
Practical work at Faculty of Science and CEITEC MU
Assessment methods
Credit after submitting and testing of protocols from all tasks.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 1999, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2023/F7810