Bi4030 Essential methods in molecular biology

Faculty of Science
Autumn 2024
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).
In-person direct teaching
Teacher(s)
Mgr. Tibor Botka, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Tibor Botka, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi4030/01_1: Mon 17:00–18:50 B11/305, T. Botka
Bi4030/01_2: Mon 17:00–18:50 B11/305, T. Botka
Prerequisites
! Bi4010c Essential mol. biol - seminar && NOW( Bi4030c Molecular biology - pract. )
Basic knowledge of general genetics, microbiology, inorganic chemistry, organic chemistry and biochemistry.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
Introduction to molecular biology methods performed in the respective practice. After completing the course, students should know the principles of methods of isolation and analysis of genetic material such as plasmid and chromosomal DNA or cloning.
Learning outcomes
The student will be able to describe the principles of molecular biological methods: plasmid DNA isolation; spectrophotometry; refractometry; ultracentrifugation; restriction endonuclease cleavage, electrophoretic separation methods; PCR; cloning including ligation and transformation; approaches to selection of transformants.
Syllabus
  • 1. Preparation of solutions and stock enzymes used in molecular biology. 2. Isolation of plasmid DNA and Determination of DNA concentration and purity. 3. Restriction analysis of nucleic acids, agarose gel electrophoresis. 4. Restriction mapping, isolation of DNA from agarose gels. 5. DNA cloning in basic types of vectors, DNA transfer to prokaryotic cells, transformation, selection of clones. 6. Centrifugation techniques. 7. Isolation of bacterial genomic DNA for DNA fingerprinting. 8. Polymerase chain reaction (PCR) and its modifications (PCR-RFLP, AP-PCR).
Literature
  • Methods in molecular biology. Totowa: Humana Press. info
  • SAMBROOK, Joseph, Edward F. FRITSCH and Tom MANIATIS. Molecular cloning : a laboratory manual. 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1989, s. v-xxxii. ISBN 0879693096. info
Teaching methods
Lectures based on explanation of the methods and presentations.
Assessment methods
Independent elaboration of exercises during the semester and passing a written test containing 20 questions (max. 50 points).
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
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