FE010 Experimental Methods in Biophysics - life science laboratory approaches and excursions

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Ctirad Hofr, Ph.D.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The objective of lectures, practical demonstrations and excursions is to acquaint with range of methods that enable us to quantitatively and qualitatively describe interactions and structures of proteins, DNA and small molecules such as drugs on one hand and methods that describe ehavior of cells and whole organisms on the other hand.
Learning outcomes
The students will learn
- principles of methods describing interactions, structure and function of biomolecules
- apply the methods and approaches to practical case studies
- use and broaden our knowledge during planned excursions to local research and development laboratories of companies e.g. TESCAN, BIOVENDOR and FEI-ThermoFisher Scientific
Syllabus
  • 1. Overview of biophysical methods and their applications
  • 2. Calorimetry of biopolymers
  • 3. UV/Vis, Infrared and Raman spectroscopy
  • 4. Fluorescence spectroscopy - FLIM , FRET, FCCS
  • 5. Fluorescence microscopy -TIRF, PLA, FRAP
  • 6. Scientific Excursion I TESCAN
  • 7. Circular dichroism of DNA a proteins
  • 8. Liquid chromatography of biomolecules
  • 9. Scientific Excursion II BIOVENDOR
  • 10. Electron microscopy
  • 11. Scientific Excursion 3 FEI-THERMOFISHER
  • 12. NMR spectroscopy of biomolecules
  • 13. Flowcytometry
  • 14. RTG crystallography
Literature
  • J.L. Nadeau: Introduction to Experimental Biophysics: Biological Methods for Physical Scientists, CRS Press 2017
Teaching methods
lecture
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2024/FE010