B6390c Methods of molecular genetics

Faculty of Science
Spring 2000
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Jana Kailerová, CSc. (seminar tutor)
prof. RNDr. Jan Šmarda, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites (in Czech)
B6130 Molecular biology || B4020 Molecular biology
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Isolation of ultrapure plasmid DNA, restriction analysis of plasmid DNA, modification of recessed termini of plasmid DNA. Agarose gel electrophoresis of plasmid DNA, elution of DNA fragments from the gel. Ligation of DNA molecules and screening of recombinant plasmids: transformation of competent bacterial cells by electroporation, minipreparation of plasmid DNA a restriction analysis. Storage of bacterial clones in deep freeze. Partial digest of DNA.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught every week.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2000/B6390c