B6721p Special Methods of Microorganisms Analysis I. - lecture

Faculty of Science
Spring 2000
Extent and Intensity
2/0/0. 0 credit(s). Type of Completion: -.
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 10 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z biochemie a molekulární biologie(B4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Use of polymerase chain reaction in gene identifications. Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
  • Enrolment Statistics (recent)
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