C6470 Advanced Methods in Biological Research

Faculty of Science
Spring 2001
Extent and Intensity
0/4/0. 5 credit(s). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Jiřina Hofmanová, CSc. (seminar tutor)
prof. RNDr. Alois Kozubík, CSc. (seminar tutor)
RNDr. Miroslav Machala, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Ladislav Dušek, Ph.D.
Prerequisites
Basic knowledge of biochemistry, cell and molecular biology are neccessary
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course gives the students an opportunity to get acquainted with a number of methods suitable for application in ecotoxicology. It includes both theoretical lectures and practical illustrations of in vivo methods (experiments with laboratory and wild animals) and in vitro methods (experiments using tissue cultures). Besides basic scientific methodology, the students will be given lectures on principles and the use of modern equipment, such as flow cytometer, confocal microscope and other methods, including molecular biology, gas and liquid chromatography, fluorimetry, spectrophotometry and luminometry.
Syllabus
  • 1. In vivo methods Experiments with laboratory animals Basic hematology methods The use of ionising irradiation Tests of toxicity 2. In vitro methods Cell cultures - Laboratory Equipments Basic methodology 3. Detection of biochemical markers Enzyme activities Oxidative stress 4. Selected molecular biology methods Flowcytometry Microscopy 5. Studies of effects of harmful substances and pharmaceuticals Cytotoxicity assays Analysis of cytokinetics 6. Working hypothesis and their verifications 7. Application in ecotoxicology and predictive oncology
Literature
  • Current Protocols in Cytometry, Ed. J. P. Robinson et al., Wiley&Sons Inc., New York 1997
  • Arachidonate related lipid mediators, Methods in Enzymology, Eds. R. C. Murphy, F. A. Fitzpatrick, Vol. 187, Academic Press, New York, 1990
  • Techniques in cell cycle analysis (Eds. J. W. Gray, Z. Darzynkiewicz), Humana Press, Clifton, New Jersey, 1987
  • Cell and tissue culture :Laboratory procedures. Insertion instructions. Edited by A. Doyle - J. B. Griffiths - D. G. Newell. Chichester: John Wiley & Sons, 1993, Volné lis. ISBN 0-471-92852-6. info
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2000, Spring 2002.
  • Enrolment Statistics (Spring 2001, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2001/C6470