C6850 Chromatographic Methods II.

Faculty of Science
Spring 2002
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Aleš Hrdlička, CSc.
Prerequisites
Knowledge of fundamental chromatographic principles and parameters corresponding to the level of introductory part of C5910 course, Chromatographic methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of capillary electrophoresis (HPCE), capillary electrochromatography (CEC), and gas chromatography (HRGC) are discussed in details.
Syllabus
  • I. Capillary electrophoresis and electrochromatography. II.1 Ion progression in electrical field, fundamental equations, terms, and parameters. II.2 Principles of CE techniques and those of capilllary electrochromatography, applications. II.3 Instrumentation. Quantitation. II. Gas chromatography. II.1 Retention characteristics, Gas flow in porous media, van Deemter equation and Golay equation. Retention indexes. II.2 Gas-Liquid Chromatography. Columns, liquid phases and their characterization, supports. II.3 Adsorption gas chromatography. Features, comparison with GLC. Adsorbents, applications. II.4 Capillary columns, packed, WCOT, PLOT, SCOT. Quality evaluation. II.5 Mobile phase, comparison of gases. II.6 Instrumentation, sample injection, detection. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
Literature
  • ROOD, D. A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems. Heidelberg: Huethig Buch Verlag, 1991. Chromatographic Methods. ISBN 3-7785-1898-4. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (Spring 2002, recent)
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