C6850 Chromatographic Methods II.

Faculty of Science
Spring 2013
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 11:00–12:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus (in Czech)
  • 1. Tenkovrstvá chromatografie. Popis experientálního uspořádání. Charakteristické rysy TLC. Fázové systémy TLC. Postupy, kroky planární chromatografie. Vyvíjení chromatogramu, Techniky řízené kapilárními salami, techniky s nuceným tokem mobilní faze. Gradientová eluce v TLC. 2. Instrumentace TLC. Konstrukce a příprava tenké vrstvy, materiály pro stacionární fáze. Vysoce účinná TLC. Dávkování vzorků, Eluční techniky, volba mobilní faze, klasifikace složek mobilní faze, modely Snyder a Nyiredy,. 3. Detekce v TLC. Přímé moetody, derivatizační postupy. Dokumentace. Vyhodnocení TLC 4. Vícerozměrná TLC. Oblasti použití TLC. 5. Hybridní metody, elektrochromatografie, micelární elektrokinetická chromatografie, mikroemulsní elektrokinetická chromatografie, kapilární chirální elektroforéza, gelová elektroforéza. Experimentální podmínky. 6. Pohyb iontu v elektrickém poli, základní rovnice, pojmy a parametry. Elektroosmotický tok. Kapilární elektroforéza (CE), Izotachoforéza, izoelektrická fokusace. Přechodová isotachoforéza. 7. Instrumentace a postupy hybridních technik. Ddávkování vzorků, Detekce 8. Inversní chromatografie. Definice, zařazení v chromatografických metodách 9. Oblasti použití inverzní chroatografie. Výstupy. Studium adsorpčních vlastností pevných materialů, měření adsorpční izotermy –Glueckauf, Gregg/Stock. Adsorpční teplo. 10. Oblasti použití inverzní chroatografie. Mezimolekulové interakce. Porozita chromatografického lože. Studium vlastností imobilizovaných polymer. Humínové kyseliny, biopolymery. Aktivní centra na povrchu pevné faze. Katalytická aktivita. Fázové přeměny. Elekrokinetické studie. 11. Kombinované techniky v separační analýze. Vícerozměrné separace. Spojení LC-GC. On-line extrakce a předseparace, SPE, SPME, SFE, ASE. Spektrální metody pro on-line detekci a identifikaci, GC-MS, LC-MS, GC(LC)-ICP-MS, LC-NMR, LC-FTIR. 12. Trendy v chromatografii. Vícerozměrová chromatografie. Vysoce rychlá chromatografie. Mikroseparace.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (Spring 2013, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2013/C6850