PřF:Bi6405 Molecular Methods - practice - Course Information
Bi6405 Methods of Molecular Biology - practice
Faculty of ScienceSpring 2004
- Extent and Intensity
- 0/3/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Jan Šmarda, CSc. (seminar tutor)
prof. Mgr. Petr Beneš, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc. - Prerequisites
- ( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology || Bi4035 Molecular biology - lab.pract. ) && NOW( Bi6400 Methods of molecular biology ) && ! B6405 Molecular Methods - practice
The course requires basic practical skils from microbiology, biochemistry and molecular biology as, for example, preparation of buffers, cultivation media, sterile liquide handling, inoculation, centrifugation and so on. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Biophysics (programme PřF, M-FY)
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Students of this course acquire experience with DNA molecule manipulations in vitro to obtain recombinant DNA molecules. The aim of the course is to teach the ways of successful purification of pure plasmid DNA from bacterial cells, its fragmentation using various restriction enzymes, modifications of DNA termini and ligation with other DNA molecules. In addition, students get experience with DNA electrophoresis and gel elution.
- Syllabus
- 1. Inoculation and cultivation of bacterial cells having appropriate plasmids. 2. Isolation of plasmid DNA from bacterial cells using Qiagen columns. 3. Restriction analysis of plasmid DNA and modification of 5 overhang termini using DNA polymerase. 4. Agarose electrophoresis of fragmented DNA. 5. Elution of appropriate fragments from a gel. 6. Ligation of appropriate DNA molecules. 7. Transformation of competent bacterial cells with ligation mixture. 8. Selection of transformants. 9. Screening of transformants for the presence of recombinant plasmid (plasmid minipreparation, restriction analysis, electrophoresis).
- Literature
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- zápočet se udílí za aktivní přístup, účast ve výuce a vypacování kvalitních protokolů z jednotlivých úloh.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Enrolment Statistics (Spring 2004, recent)
- Permalink: https://is.muni.cz/course/sci/spring2004/Bi6405