PřF:Bi2050c General microbiology pr. - Course Information
Bi2050c General microbiology practice
Faculty of ScienceSpring 2006
- Extent and Intensity
- 0/4/0. 4 credit(s). Type of Completion: z (credit).
- Teacher(s)
- RNDr. Ludmila Tvrzová, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Danuška Horáková, CSc. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Course objectives
- The aim of the practical course of general microbiology are basic methods used in microbiological laboratory (methods of sterile inoculation and cultivation, slides preparation and microscopic examination of microorganisms, determination of the number of bacteria in different samples, methods of work with bacteriophage, yeasts and microscopic fungy).
- Syllabus
- 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of Microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition 5. Determination of number of bacteria by cultivation method 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Determination of the number of phage particles in lysate of bacterial cells 10. Yeasts, test of vitality 11. Microorganisms in soil. Inoculation of microscopic fungi. 12. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negativ - staining, capsule - staining. Microscopic examination of endospores. 13. Microscopic fungi. 14. Protocols, test and unknown strain microscopic examination.
- Literature
- JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
- Assessment methods (in Czech)
- praktická cvičení, zápočet
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Enrolment Statistics (Spring 2006, recent)
- Permalink: https://is.muni.cz/course/sci/spring2006/Bi2050c