Bi8202c Proteomics - practice

Faculty of Science
Spring 2006
Extent and Intensity
0/3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Mgr. Pavel Mazura, Ph.D. (seminar tutor)
Mgr. Ivana Urbánková (seminar tutor)
Bc. Martina Válková (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Timetable of Seminar Groups
Bi8202c/Beh_1: No timetable has been entered into IS. R. Dopitová, J. Hejátko, H. Konečná, P. Mazura, E. Nejedlá, E. Paděrová, I. Urbánková, M. Válková, Z. Zdráhal
Bi8202c/Beh_2: No timetable has been entered into IS. R. Dopitová, J. Hejátko, H. Konečná, P. Mazura, E. Nejedlá, E. Paděrová, I. Urbánková, M. Válková, Z. Zdráhal
Prerequisites
A prerequisite for a successful passing out a course is knowledge of basics of biochemistry and molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
In the course the students will be educated in the basics of the experimental approaches recently used in the studies of the function of the sum of all proteins of the respective organism (proteome), but on the other hand also to the study of the features of individual proteins. Students will practice the methods of protein expression and purification in heterologous expression systems (E. coli), ranging from the recombinant DNA isolation and characterization, through the protein isolation, purification and purity analysis using SDS-PAGE and image analysis to the structural and functional analysis of purified proteins. Further there will be performed total protein isolation from the model organism Arabidopsis thaliana and students will be educated in the basic approaches of the proteome analysis using 2D electrophoresis and its evaluation using image analysis. It will be followed by the practical demonstration of the basics of the protein identification using MS spectrometry and the basics of the work with protein databases. All the above mentioned methods and methodological approaches are used in the Laboratory of Plant Molecular Physiology used for the solution of real biological and biochemical problems by the staff and students of the laboratory that will participate on the teaching of the course.
Assessment methods (in Czech)
Pro úspěšné absolvování kurzu a udělení zápočtu bude požadováno splnění všech studijních povinností a to nejpozději v POSLEDNÍ DEN konání daného běhu (tedy vždy v pátek). Jedná se především o absolvování několika písemných testů a odevzdání VŠECH požadovaných protokolů.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.
  • Enrolment Statistics (Spring 2006, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2006/Bi8202c