Bi9042 Analysis of chromatin structure - practical training

Faculty of Science
Spring 2007
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Jiří Fajkus, CSc. (seminar tutor)
Mgr. Eva Sýkorová, CSc. (seminar tutor)
Mgr. Tatiana Vrtíková Kubičárová, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi9041 Struct. euk. chromosomes )
Basic knowledge of molecular biology and biochemistry, parallel or previous subscription of the course Structure and function of eukaryotic chromosomes, knowledge of laboratory safety instructions
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course represents practical training to the course Structure and function of eukaryotic chromosomes. The aim of the course is to provide students with practical experience with methods used in analysis of the structure of chromosomes and chromatin.
Syllabus
  • 1. Isolation of cell nuclei from animal and plant tissues 2. Digestion of nuclei with micrococcal nuclease 3. Extraction of nucleosomal DNA 3. Analysis of nucleosomal DNA by electrophoresis 4. Preparation of mononucleosomal DNA from agarose gel 5. Mapping of nucleosome position using restriction enzymes or primer extension 6. Computer modelling of nucleosome position 7. Comparison of experimental and computer-predicted results 8. Extraction of chromatin proteins 9. Analysis of chromatin proteins by SDS-PAGE
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.
  • Enrolment Statistics (Spring 2007, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2007/Bi9042