PřF:Bi4091c Pract. from gener. microbiol. - Course Information

Bi4091c Practise from general microbiology

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Mgr. Andrea Vávrová, Ph.D. (seminar tutor)

Guaranteed by

RNDr. Ludmila Tvrzová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Ludmila Tvrzová, Ph.D.

Timetable of Seminar Groups

Bi4091c/01: Thu 15:00–17:50 Bpb,02012, M. Krsek, A. Vávrová
Bi4091c/02: Thu 8:00–10:50 Bpb,02012, M. Krsek, A. Vávrová

Prerequisites (in Czech)

NOW( Bi4090 General microbiology )

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20

fields of study / plans the course is directly associated with

• General Biology (programme PřF, B-BI, specialization Microbiology)

Course objectives

At the end of this course, students should be able to work aseptically, to inoculate and cultivate bacteria, to prepare stained and native microscopic slides, to determinate of the number of bacteria in different samples, to determine indicators of microbial water quality, to use some basic identification methods.

Syllabus

• 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of viable bacteria by plate count 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Determination of the number of phage particles in lysate of bacterial cells 10. Yeasts, test of vitality 11. Microorganisms in soil. Inoculation of microscopic fungi. 12. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negative - staining, capsule - staining. Microscopic examination of endospores. 13. Microscopic fungi. 14. Reports, test and microscopic examination/determination of unknown bacterial strain.

Literature

• JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info

Assessment methods

practical classes, reports, written test, credit

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• Bi2060c Basic microbiology - practice
  NOW(Bi2060) && !NOWANY(Bi4091c, Bi5710c, B4090c) && !Bi4091c && !Bi5710c && !Bi4090c  
• Bi4090c General microbiology - practical course
  NOW(Bi4090) && !NOWANY(Bi4091c, Bi5710c, Bi2060c) && !Bi4091c && !Bi5710c && !Bi2060c && rocnik(2)  
• Bi5710c Microbiology - practice
  NOW(Bi5710) && !NOWANY(Bi4091c, Bi4090c, Bi2060c) && !Bi4091c && !Bi4090c && !Bi2060c && rocnik(2) || rocnik(3)  

• Enrolment Statistics (Spring 2009, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2009/Bi4091c