C8160 Enzymology

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Thu 14:00–15:50 B09/316
Prerequisites
C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry, organic chemistry and physical chemistry are recommended.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
An advanced lecture. Enzyme nomenclature. Measurement of enzyme activity. Isolation and storage of enzymes. Mechanisms of enzyme catalysis. Enzyme kinetics. The effect of temperature, pH and inhibitors. Techniques of enzyme immobilization, the effects of immobilization on enzyme properties. Use of enzymes in biochemical analyses,molecular biology, medicine and technology.
Syllabus
  • 1) First sight into the world of enzymes: structure, specifity, nomenclature. 2) Enzyme activity and their measurement. 3) Isolation of enzymes from the natural sources and expression systems. 4) Enzyme catalysis from the point of view of organic chemistry. 5) Enzyme catalysis from the point of view of physical chemistry. 6) Basic theoretical concepts in enzyme kinetics. 7) Effects of environmental factors (pH, temperature) on enzymes. 8) Reversible and irreversible inhibition of enzymes. 9) Kinetics of multisubstrate enzyme reactions. 10) Cooperativity in enzyme function. 11) Immobilized enzyme systems. 12) Enzymes as tools in bioanalysis. 13) Use of enzymes in technology and medicine. Enzyme engineering.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
  • KOTYK, Arnošt and Jaroslav HORÁK. Enzymová kinetika. Vyd. 1. Praha: Academia, 1977, 268 s. info
Assessment methods
A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2009/C8160