C6290 Atomic Absorption Spectrometry

Faculty of Science
Spring 2010
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Josef Komárek, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Josef Komárek, DrSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Thu 11:00–11:50 AK1
Prerequisites
Knowledge of Analytical chemistry on the level of fundamental courses.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 15 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to: - understand the problems in atomic absorption spectrometry (AAS) - outline parameters important for measurement in AAS - choose system of elimination of interferences and background correction - compare capacities of flame AAS, AAS with electrothermal atomization and AAS with generation of volatile compounds - propose suitable analytical procedure for practical application
Syllabus
  • 1. Basic principles, absorption of free atoms, atomic line widths, resonance line.
  • 2. Instrumentation, light sources, hollow cathode lamp, electrodeless discharge lamp.
  • 3. Spectral interferences.
  • 4. Background correction with a continuum source.
  • 5. Background correction by the Zeeman effect and Smith-Hieftje method.
  • 6. Flames, burners, nebulizers, microsampling boat, Delves cup system, STAT, FIA.
  • 7. Atomization in flames, nebulization, vaporization, chemical reactions.
  • 8. Transport, vaporization, gas phase and spatial distribution interferences, their elimination and control.
  • 9. Electrothermal atomizers, electrographite, pyrolytic graphite, tungsten.
  • 10. Graphite furnaces, WETA, platform technique, graphite probe.
  • 11. Electrothermal atomization, mechanisms, interferences.
  • 12. Matrix modifiers, effect of organic solvents.
  • 13. Generation of volatile hydrides, atomization, interferences.
  • 14. Cold vapour mercury system.
Literature
  • KOMÁREK, Josef. Atomová absorpční spektrometrie (Atomic absorption spectrometry). Brno: Masarykova univerzita v Brně, 2000, 85 pp. ISBN 80-210-2500-X. info
  • Welz B., Sperling M.: Atomabsorptionsspektrometrie. Wiley-VCH, Wienheim 1997.
  • HASSAN, Saad S. M. Organic analysis using atomic absorption spectrometry. Chichester: Ellis Horwood Limited, 1984, 384 s. ISBN 0-85312-559-7. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of basic principles of atomic absorption spectrometry, atomization in atomizers, interferences, their elimination, background correction and the use in practical analysis is emphasized.
Assessment methods
Final assessment (at the end of semester) is by oral examination. The exam consists in four questions, which require description and explanation of asked topic.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2010, recent)
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