Bi6040c Plant physiology for advanced students I. - practice

Faculty of Science
Spring 2011
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Milan Baláž, Ph.D. (seminar tutor)
prof. Ing. Miloš Barták, CSc. (seminar tutor)
RNDr. Jaroslava Dubová, CSc. (seminar tutor)
doc. RNDr. Vít Gloser, Ph.D. (seminar tutor)
doc. RNDr. Marie Kummerová, CSc. (seminar tutor)
Mgr. Peter Váczi, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jan Gloser, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Prerequisites (in Czech)
NOW( Bi6040 Plant. physiol. adv. I. )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this advanced course students are capable to:
apply some techniques of image analysis;
monitor processes connected with exchange of compounds between plant and the environment;
measure rates of important metabolic processes in plants by non-destructive methods;
evaluate impact of stress factors on plant functioning.
Syllabus
  • Methods of image processing and analysis.
  • Thin layer chromatography of photosynthetic pigments and preparation of standards.
  • Measurement of root respiration by oxymetric method, analysis of respiratory pathways.
  • Isolation of leaf chloroplasts, tests of their activity.
  • Advanced methods in measurement of plant gas exchange.
  • Measurement of hydraulic resistance of xylem and the degree of xylem integration.
  • The effects of toxic compounds on the activity of Hill reaction in chloroplasts.
  • Effects of quantity and quality of light on the growth of plants in in vitro cultures.
  • Measurements of the rate of photosynthetic processes by oxymetry and fluorometry – comparison of methods.
  • Ascorbateperoxidase – determination of activity and the amount of protein.
  • Detection of stress due to exposure to toxic compounds using fluorometric methods.
Literature
  • BUCHANAN, Bob, Wilhelm GRUISSEM and Russell JONES. Biochemistry & molecular biology of plants. Rockville, Maryland: American society of plant physiologists, 2000, 1367 pp. ISBN 0-943088-39-9. info
  • HOLME, David J. and Hazel PECK. Analytical biochemistry. 3rd ed. Essex: Longman, 1998, xiii, 488. ISBN 058229438X. info
  • HELDT, Hans-Walter. Plant biochemistry. Edited by Fiona Heldt. 3rd ed. Amsterdam: Elsevier Academic Press, 2005, xxvi, 630. ISBN 0120883910. info
  • Plant biochemistry. Edited by Prakash M. Dey - J. B. Harborne. San Diego: Academic Press, 1997, viii, 554. ISBN 0122146743. info
  • NOBEL, Park S. Physicochemical & environmental plant physiology. 2nd ed. San Diego: Academic Press, 1999, xxiv, 474. ISBN 0125200250. info
Teaching methods
Laboratory course.
Assessment methods
Evaluation of each topic will be based on written presentation of results.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2011/Bi6040c