Bi6420c Microbial Ecotoxicology - practical course

Faculty of Science
Spring 2011
Extent and Intensity
0/2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Jakub Hofman, Ph.D. (seminar tutor)
Mgr. Chachchaya Cervinka (assistant)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: prof. RNDr. Jakub Hofman, Ph.D.
Timetable
Thu 9:00–12:50 Kontaktujte učitele
Prerequisites
NOW( Bi6420 Microbial Ecotoxicology )
It is required to visit Ecotoxicology of microorganisms parallely
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This course comprises from laboratory exercises. At the end, students should be able to:
- measure and evaluate main soil microbial parameters - microbial biomass and its activities: basal respiration, substrate induced respiration, potential ammonification, potential nitrification
- compute and interpret combined eco-physiological indices
- compare the results for soil with different effects of contamination
- explain observed differences
- judge overall changes of soil biological quality caused by contamination
- work in team in the laboratory and generate together report from the exercises
Syllabus
  • During week laboratory course, students will measure range of microbial parameters for soil contaminated with cadmium at two concentrations. At the end they compare these results with un-contaminated soil and interpret the influece of cadmium.
  • During the exercises, students will measure:
  • - microbial biomass with fumigation - extraction method
  • - basal and substrate induced respiration using gas chromatograph
  • - potential nitrification by potential ammonium oxidation
  • - potential ammonification with arginine test
Literature
  • ATLAS, Ronald M. Handbook of media for environmental microbiology. Boca Raton: CRC Press, 1995, iv, 540 s. ISBN 0-8493-0603-5. info
  • Methods in applied soil microbiology and biochemistry. Edited by Kassem Alef - Paolo Nannipieri. London: Academic Press, 1995, 576 s. ISBN 0125138407. info
Teaching methods
Laboratory exercises running as almost independent laboratory work of students controlled and supervised by lecturer. Attendance in experimental work is mandatory. Work in the laboratory follows exact rules (manual to exercises) given by lecturer at the course beginning. Students work in teams and generate together also final reports.
Assessment methods
For credits it is mandatory: 1) be present in the course, 2) be activelly participating during the laboratory work, 3) provide final protocols (reports) containing overview of the methods used, gained results (measured and calculated indices), interpretation of the results.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015.
  • Enrolment Statistics (Spring 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2011/Bi6420c