PřF:FA603 Electron microscopy in biol. - Course Information

FA603 Electron microscopy in biology

Extent and Intensity

1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

Ing. Jana Nebesářová, CSc. (lecturer)

Guaranteed by

prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: prof. Dr. Jiří Kozelka, PhD.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

The course introduces basic physical principles of transmission and scanning electron microscopy, and preparation methods of biological objects for electron microscopy.

Syllabus

• Physical principles of electron microscopy and physical properties of accelerated electrons, resolution, magnification
• Transmission electron microscope, electromagnetic lenses and defects, construction of the microscope
• Preparation of specimens for TEM, physical methods, cryomethods, microwaves
• Preparation of specimens for TEM, chemical methods, fixation, dehydration, embedding
• Preparation of ultra-light cuts, ultramicrotome, knives, contrasting, underlying films
• Scanning electron microscopy, construction of the microscope
• Preparation of specimens for SEM, drying, coating
• Electron microscopy cytology
• Artifacts caused by TEM and SEM preparation, by microscope, photographic process
• Microscopic images, digital and classical photography

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: every week.

• Enrolment Statistics (Spring 2011, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2011/FA603