Bi5620c Ecotoxicological Bioassays - practical course

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
0/0/2. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jiří Novák, Ph.D. (seminar tutor)
RNDr. Kateřina Nováková, Ph.D. (seminar tutor)
doc. Mgr. Klára Hilscherová, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. Mgr. Klára Hilscherová, Ph.D.
Supplier department: RECETOX – Faculty of Science
Prerequisites (in Czech)
NOW( Bi5620 Ecotoxicological Bioassays )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to:
- Design, plan and carry out the ecotoxicological evaluation of hazardousness of various materials (chemical compounds, wastes, contaminated environmental matrices)
- Arrange a battery of biotests according to the goals of the assessment
- Design the optimal test arrangement to minimize the cost and number of needed model organism and maximize the gained information
- Conduct the ecotoxicological biotest with model organisms on different trophic levels (producents, consuments and destruents) according to standard guidelines
- Appraise the validity and representativeness of data obtained from ecotoxicological tests
- Predict the toxicity of various materials/compounds on the base of the results of ecotoxicological biotests
- Assess the limitations of the tests in extrapolation of the results to other species
- Discuss the practical aspects of ecotoxicological biotests
- Analyze and interpret the biotest results also from other institutions
- Prepare standard reports of the ecotoxicological assessment according to the legal and guideline requirements
Syllabus
  • 1) Introduction – needs of ecotoxicological testing, requirements for evaluation of ecotoxicity of stressors, ethical aspects
  • 2) Tests with representatives of different trophic levels, special biotests, selection and set up of battery of biotests
  • 3) Test design, planning and realization of biotests, practical aspects
  • 4) Legal context, standardized tests – guideline system CSN, ISO, OECD
  • 5) Test of ecotoxicity for producents I. – test of growth inhibition of green algae
  • 6) Test of ecotoxicity for producents II. - test of inhibition of germination and growth of macrophytes
  • 7) Test of ecotoxicity for destruents – acute toxicity test with luminiscent bacteria (Microtox)
  • 8) Test of ecotoxicity for consuments – test of immobilization of Daphnia magna
  • 9) Test of ekotoxicity of soil + practical aspects of tests for solid matrice
  • 10) In vitro tests for evaluation of specific effects of contaminants
  • 11) Tests of ecotoxicity with vertebrates – demonstration of fish toxicity tests
  • 12) Microbiotests – fast tests of second generation
  • 13) Preparation of standardized reports from tests, GLP and validity requirements
  • 14) Evaluation, analysis and interpretation of the measured data
Literature
  • Rand, G.M. Fundamentals of aquatic toxicology. Taylor and Francis, 1995, Washington D.C.
  • Ecotoxicology : a hierarchical treatment. Edited by Michael C. Newman - Charles H. Jagoe. Boca Raton: Lewis Publishers, 1996, 411 s. ISBN 0-56670-127-9. info
  • CALOW, P. Handbook of Ecotoxicology Vol. I and II. London, U.K.: Blackwell Scientific publications, 1993. info
Teaching methods
The class consists of theorethical introductory part and set of practical laboratory exercises in small groups. The learning activities include individual home study of the methodology and theoretical preparation for the biotests, practical realization of the biotests in laboratory, dala analysis and preparation of detailed protocols from each biotest.
Assessment methods
Requirements for the credit: active presence on all lab exercises, data analysis of the tests results, elaboration of detailed protocols for every conducted biotest
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.